Affinity capture in bottom-up protein analysis – Overview of current status of proteolytic peptide capture using antibodies and molecularly imprinted polymers

Antibody-based affinity capture has become the gold standard in sample preparation for determination of low-abundance protein biomarkers biological matrices prior to liquid chromatography-mass spectrometry (LC-MS) determination. This comprises both intact proteins digestion step and proteolytic peptides after sample. The latter can be performed using antibodies specifically developed target peptides, as well by less explored use anti-protein epitope peptide. Molecularly imprinted polymers (MIPs), also called plastic are another affinity-based approach emerging technique LC-MS based biomarker analysis. current review gives a critical comprehensive overview peptide MIPs during last five years. main emphasis is on non-modified while brief containing post-translational modifications (PTMs) provided.